• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The disclosure relates to the technical field of bacterial manure, in particular, to a mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil and a preparation method thereof. The specific components of the seed coating agent are: 1-1.5 parts of arbuscular mycorrhiza fungi preparation, 0.1-0.2 parts of trichoderma harzianum preparation, 3-5 g of xanthan gum, l-l.2g of calcium carbonate, 0.1-0.2g of citric acid aqueous solution, 0.2-0.3 g of ethyl lactate, and 80—100mg of polylactic acid. In the disclosure, a microcapsule is prepared by forming double emulsions with the use of polylactic acid, xanthan gum, etc.; the prepared seed coating agent has good membrane forming property and high embedding rate, with good stability of the formed wall, so as to effectively protect the activity of fungi agent, to directly coat the seed and have a good sustained-release effect, through the synergy between the arbuscular mycorrhiza fungi and the trichoderma harzianum, the success rate of inoculation is high, and after inoculation, the output of crops is significantly increased and the incidence of disease for crops is reduced.
    公开号:NL2025603A
    申请号:NL2025603
    申请日:2020-05-18
    公开日:2021-07-28
    发明作者:Tang Chaohui；Wan Shubo；Guo Feng；Zhang Jialei；Liu Ping；Cui Li；Wang Jianguo；Li Xinguo；Yang Sha；Meng Jingjing；Geng Yun；Li Qingkai
    申请人:Biotechnology Res Ct Shandong Academy Agricultural Sciences；
    IPC主号:
    专利说明:

    [0001] [0001] The disclosure relates to the technical field of bacterial manure, in particular, to a mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil and a preparation method thereof. Background
    [0002] [0002] Mycorrhizal is a common symbiosis of plants in nature, especially a symbiosis formed by the mycorrhizal fungi and roots of higher plants in soil. Arbuscular mycorrhiza fungi (AMF) are a type of heterotrophic microorganisms widely distributed in farmland ecosystems, and account for 5-50% of the microbial biomass in soil. The arbuscular mycorrhiza fungi can form symbiont with 80% of the plants on the land. They are very important types of functional microorganisms in the soil, which have an important impact on plant growth and development and the structural function of plant communities. The formation of plant mycorrhiza significantly improves, for the host plant, the absorption of nutrients and resistance to drought and high salt stress under conditions of low phosphorus, drought, and high salt. Studies have shown that inoculation of AMF under certain conditions can promote the absorption and utilization of phosphorus, zinc, and copper in the soil by plants, and can also have a certain effect on the absorption of nitrogen, potassium, magnesium, and sulfur.
    [0003] [0003] At present, the low effective utilization rate of fertilizers such as N, P and K in China has caused a waste of resources, and also the long-term application of a large number of fertilizers has deteriorated the soil quality and decreased the fertility, which further has led to the deterioration of the quality of agricultural products and serious environmental pollution. And, the soil salinization has become a worldwide problem. China has large and widely-distributed saline-alkali lands, and the problem of salinization for arable lands is becoming more and more serious. How to improve the utilization rate of chemical fertilizers, improve soil fertility, and improve the structure of microbial community in saline-alkali soils has become an urgent problem to be solved. How to reasonably improve the use of saline-alkali lands is also of great significance to the quality and output of the agricultural products in China.
    [0004] [0004] In view of the difficulties in improving saline-alkali land, imbalance of microbial flora, poor fertilizer utilization, etc., the disclosure provides a mycorrhizal dry-powder seed coating agent for improving microbial community structure in saline- alkali soil.
    [0005] [0005] The disclosure further provides a preparation method for a mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil.
    [0006] [0006] In order to achieve above purpose, the disclosure employs the following technical solutions: The disclosure provides a mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil, which is composed of ingredients of, by weight, 1-1.5 parts of arbuscular mycorrhiza fungi preparation, 0. 1-0.2 parts of trichoderma harzianum preparation, 3-5g of xanthan gum, 1-1.2g of calcium carbonate,
    [0007] [0007] The polylactic acid used in the disclosure has a molecular weight of 2000-3000.
    [0008] [0008] The disclosure further provides a preparation method for the above mycorrhizal microcapsule seed coating agent, which includes steps of: (1) first dissolving the polylactic acid in the ethyl acetate to obtain a mixed solution of polylactic acid-ethyl acetate, after mixing well, adding the arbuscular mycorrhiza fungi preparation and the trichoderma harzianum preparation to the mixed solution, and then mixing well to form an initial emulsion; (2) then adding the initial emulsion with the xanthan gum, the calcium carbonate, the citric acid aqueous solution and the ethyl lactate for stirring at 35-40°C for 24h to obtain a composite emulsion, and then adding a sodium chloride aqueous solution and stirring at room temperature for 2min; (3) drying the stirred product under vacuum to obtain an arbuscular mycorrhiza microcapsule seed coating agent.
    [0009] [0009] Further, a material-liquid ratio of the polylactic acid and the ethyl acetate is 1 mg: 1 mL.
    [0010] [0010] Further, a volume ratio of the composite emulsion and the sodium chloride aqueous solution is 1: 5.
    [0011] [0011] Further, the sodium chloride aqueous solution has a concentration of 4.5 g/L.
    [0012] [0012] Further, in the step (1), the stirring has a rate of 7000-7500 r/min.
    [0013] [0013] Further, in the step (2), the stirring has a rate of 5000-5500 r/min. The arbuscular mycorrhiza fungal strain used in the disclosure is Glomusmosseae, which is commercially available; the trichoderma harzianum strain used in the disclosure is purchased from the China General Microbiological Culture Collection Center (CGMCC).
    [0014] [0014] The disclosure has the following beneficial effects: (1) In the disclosure, a microcapsule is prepared by forming double emulsions with the use of polylactic acid, xanthan gum, etc.; the prepared seed coating agent has good membrane forming property and high embedding rate, with good stability of the formed wall, so as to effectively protect the activity of fungi agent; (2) The coating agent prepared by the disclosure may directly coat the seed and have a good sustained-release effect; through the synergy between the arbuscular mycorrhiza fungi and the trichoderma harzianum, the success rate of inoculation is high, and after inoculation, the output of crops is significantly increased and the incidence of disease for crops is reduced. Embodiments
    [0015] [0015] The technical solutions of the disclosure will be further described in detail below through specific embodiments.
    [0016] [0016] Embodiment 1 A mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil is prepared by 1 part of arbuscular mycorrhiza fungi preparation, 0.1 part of trichoderma harzianum preparation, 3g of xanthan gum, 1.2g of calcium carbonate, 0.1g of citric acid aqueous solution, 0.2g of ethyl lactate, and 80mg of polylactic acid (molecular weight of 2000).
    [0017] [0017] The preparation method is as follows: (1) first dissolving the polylactic acid in the ethyl acetate of 80mL to obtain a mixed solution of polylactic acid-ethyl acetate, after mixing well at 7000r/min for 2min, adding the arbuscular mycorrhiza fungi preparation and the trichoderma harzianum preparation to the mixed solution, and then mixing well at 7000r/min for 2min to form an initial emulsion;
    [0018] [0018] Embodiment 2 A mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil is prepared by 1.2 part of arbuscular mycorrhiza fungi preparation, 0.2 part of trichoderma harzianum preparation, 4g of xanthan gum, 1.2g of calcium carbonate, 0.2g of citric acid aqueous solution, 0.3g of ethyl lactate, and 100mg of polylactic acid (molecular weight of 3000).
    [0019] [0019] The preparation method is the same as Embodiment 1.
    [0020] [0020] Embodiment 3 A mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil is prepared by 1.5 part of arbuscular mycorrhiza fungi preparation, 0.2 part of trichoderma harzianum preparation, 5g of xanthan gum, 1.0g of calcium carbonate, 0.2g of citric acid aqueous solution, 0.3g of ethyl lactate, and 90mg of polylactic acid (molecular weight of 2500).
    [0021] [0021] The preparation method is the same as Embodiment 1.
    [0022] [0022] Comparative Example 1 A mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil is prepared by 1.2 parts of arbuscular mycorrhiza fungi preparation, 0.2 parts of trichoderma harzianum preparation, 4g of xanthan gum, 1.2g of calcium carbonate, 0.2g of citric acid aqueous solution, and 0.3g of ethyl lactate.
    [0023] [0023] The preparation method is as follows: (1) first adding the arbuscular mycorrhiza fungi preparation and the trichoderma harzianum preparation to the ethyl acetate, and then mixing well at 7000r/min for 2min to form a suspension, (2) then adding the suspension with the xanthan gum, the calcium carbonate, the citric acid aqueous solution and the ethyl lactate for stirring at 35-40°C for 24h to obtain an emulsion, and then adding a sodium chloride aqueous solution with a concentration of
    [0024] [0024] Comparative Example 2 A mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil is prepared by 1.4 parts of arbuscular mycorrhiza fungi preparation, 4g of xanthan gum, 1.2g of calcium carbonate, 0.2g of citric acid aqueous solution, 0.3g of ethyl lactate, and 100mg of polylactic acid (molecular weight of 3000).
    [0025] [0025] The preparation method is the same as Embodiment 1.
    [0026] [0026] Comparative Example 3 A preparation method for a mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil is prepared by a method same as Embodiment 1.
    [0027] [0027] The preparation method is as follows: (1) first dissolving the polylactic acid in the ethyl acetate of 80mL to obtain a mixed solution of polylactic acid-ethyl acetate, after mixing well at 7000r/min for 2min, adding the arbuscular mycorrhiza fungi preparation and the trichoderma harzianum preparation to the mixed solution, and then mixing well at 7000r/min for 2min to form an initial emulsion; (2) then adding the initial emulsion with the xanthan gum, the calcium carbonate, the citric acid aqueous solution and the ethyl lactate for stirring at 35-40°C for 24h to obtain a composite emulsion, and stirring at room temperature at 5000r/min for 2min; (3) drying the stirred product under vacuum to obtain an arbuscular mycorrhiza microcapsule seed coating agent.
    [0028] [0028] Comparative Example 4 A preparation method for a mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil is prepared by a method same as Embodiment 1.
    [0029] [0029] The preparation method is as follows: (1) first dissolving the polylactic acid in the ethyl acetate of 80mL to obtain a mixed solution of polylactic acid-ethyl acetate, then adding the xanthan gum, the calcium carbonate, the citric acid aqueous solution and the ethyl lactate, and after mixing well at 7000r/min for 2min, adding the arbuscular mycorrhiza fungi preparation and the
    [0030] [0030] Comparative Example 5 A mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline-alkali soil 1s prepared by 1.2 parts of arbuscular mycorrhiza fungi preparation, 0.2 parts of trichoderma harzianum preparation, 1.2g of calcium carbonate, 0.2g of citric acid aqueous solution, 0.3g of ethyl lactate, and 100mg of polylactic acid (molecular weight of 3000).
    [0031] [0031] The preparation method is as follows: (1) first dissolving the polylactic acid in the ethyl acetate of 80mL to obtain a mixed solution of polylactic acid-ethyl acetate, after mixing well at 7000r/min for 2min, adding the arbuscular mycorrhiza fungi preparation and the trichoderma harzianum preparation to the mixed solution, and then mixing well at 7000r/min for 2min to form an initial emulsion; (2) then adding the initial emulsion with the calcium carbonate, the citric acid aqueous solution and the ethyl lactate for stirring at 35-40°C for 24h to obtain a composite emulsion, and then adding a sodium chloride aqueous solution with a concentration of
    [0032] [0032] Effect Embodiment (1) The embedding rate and the particle size of the microcapsules prepared in Embodiments 1-3 and Comparative Examples 1-5 are tested, wherein the embedding rate is determined by indirect method, and the particle size is detected by a particle size detector; the specific results are shown in Table 1.
    [0033] [0033] Table 1 ee pen
    [0034] [0034] Table 2 Frequency of Intensity of Abundance of clump mycorrhizal infection |mycorrhizal infection (planting (A)/% (F)/% (M)/% Embodimen 93.5 95.1 80.2 79.9 78.1 76.8
    [0035] [0035] Table 3 ee Dm Comparative Example 37.3 271.9 ee ee Comparative Example 39.1 290.7 ee ee 3
    [0036] [0036] Table 4 Re eee Comparative Example|1.58 28.4 — Comparative Example|1.52 29.9 ee Comparative Example/1.41 22.1 ee Comparative Example|1.53 30.1 en Oe Comparative Example|1.49 27.8 en ee
    权利要求:
    Claims (8)
    [1]
    1. Mycorrhizal microcapsule seed coating agent for improving microbial community structure in saline alkaline soil, composed of ingredients of 1-1.5 parts preparation of arbuscular mycorrhizal fungi, 0.1-0.2 parts preparation of trichoderma harzianum, 3 -5 g xanthan gum, 1-1.2 g calcium carbonate, 0.1-0.2 g aqueous citric acid solution, 0.2-0.3 g ethyl lactate and 80-100 mg polylactic acid.
    [2]
    The mycorrhizal microcapsule seed coating agent according to claim 1, wherein the polylactic acid has a molecular weight of 2000-3000.
    [3]
    A production method for the mycorrhizal microcapsule seed coating agent according to claim 1 or 2, comprising the steps of: (1) first dissolving the polylactic acid in the ethyl acetate to obtain a mixed solution of polylactic acid-ethyl acetate, after mixing well, adding the preparation of arbuscular mycorrhizal fungi and the preparation of trichoderma harzianum to the mixed solution and then mixing well to form an initial emulsion; (2) then adding the xanthan gum, the calcium carbonate, the aqueous citric acid solution and the ethyl lactate to the initial emulsion for stirring at 35-40 °C for 24 hours to obtain a compound emulsion, and then adding an aqueous sodium chloride solution and stirring for 2 min at room temperature; (3) drying the stirred product under vacuum to obtain an arbuscular mycorrhizal microcapsule seed coating agent.
    [4]
    The production method according to claim 3, wherein a material-liquid ratio of the polylactic acid and the ethyl acetate is 1 mg: 1 ml.
    [5]
    The method of claim 3, wherein a volume ratio of the composite emulsion and the aqueous sodium chloride solution is 1:5.
    S11 -
    [6]
    The preparation method according to claim 5, wherein the aqueous sodium chloride solution has a concentration of 4.5 g/l.
    [7]
    The preparation method according to any one of claims 3 to 6, wherein in step (1) the stirring is at a speed of 7000-7500 rpm.
    [8]
    The preparation method according to claim 7, wherein in step (2) the stirring has a speed of 5000-5500 rpm.
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